Difference between revisions of "Part:BBa K4586013"

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This part is implicated in our design to increase the amount of our therapeutic agent within the exosomes, as the CX43 transmembrane portion facilitates the loading of our cargo into the exosomes.
 
This part is implicated in our design to increase the amount of our therapeutic agent within the exosomes, as the CX43 transmembrane portion facilitates the loading of our cargo into the exosomes.
 +
==Literature Characterization==
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The study made western blot analyses of extracts from various cell lines to find out the effect of connexin 43 in different cells.
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<html><div align="center"style="border:solid #17252A; width:50%;float:center;"><img style="                              max-width:850px;
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width:75%;
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height:auto;
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position: relative;
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top: 50%;
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left: 35%;
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transform: translate( -50%);
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padding-bottom:25px;
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padding-top:25px;
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"src="https://static.igem.wiki/teams/4586/wiki/literature-characterisation-parts/cx43.png">
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<p class=MsoNormal align=center style='text-align:left;border:none;width:98% ;justify-content:center;'><span
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lang=EN style='font-size:11.0pt;line-height:115%'>By using Connexin 43 (E7N2R) XP Rabbit mAb in the upper and GAPDH (D16H11) XP Rabbit mAb in the lower. Connexin 43 protein is expressed at low levels in BeWo cells, which is compatible with the expected expression pattern.
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</span></p></div></html>
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<br><br><br><br>
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<html><div align="center"style="border:solid #17252A; width:50%;float:center;"><img style="                              max-width:850px;
 +
width:75%;
 +
height:auto;
 +
position: relative;
 +
top: 50%;
 +
left: 35%;
 +
transform: translate( -50%);
 +
padding-bottom:25px;
 +
padding-top:25px;
 +
"src="https://static.igem.wiki/teams/4586/wiki/literature-characterisation-parts/3.png">
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<p class=MsoNormal align=center style='text-align:left;border:none;width:98% ;justify-content:center;'><span
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lang=EN style='font-size:11.0pt;line-height:115%'>In lane 1, we have Western blot analysis of extracts from control 293T cells; in lane 2, we have Western blot analysis of connexin 43 knockout 293T cells, and that was done using Connexin 43  XP Rabbit mAb in the upper and GAPDH  XP Rabbit mAb  in the lower.
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Connexin 43 antibody specificity is demonstrated by the absence of a signal in connexin 43 knockout 293T cells.
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</span></p></div></html>
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==References==
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Giepmans, B.N. et al. (2001) J Biol Chem 276, 8544-9.
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 05:04, 25 September 2023


CX43

Part Description

This part is a transmembrane protein called connexin (CX43) that oligomerizes to form hexameric channels, called hemichannels, that can control the interaction and transfer of information between exosomes and cells of interest, which is called intercellular communication.

Usage

This part is implicated in our design to increase the amount of our therapeutic agent within the exosomes, as the CX43 transmembrane portion facilitates the loading of our cargo into the exosomes.

Literature Characterization

The study made western blot analyses of extracts from various cell lines to find out the effect of connexin 43 in different cells.

By using Connexin 43 (E7N2R) XP Rabbit mAb in the upper and GAPDH (D16H11) XP Rabbit mAb in the lower. Connexin 43 protein is expressed at low levels in BeWo cells, which is compatible with the expected expression pattern.





In lane 1, we have Western blot analysis of extracts from control 293T cells; in lane 2, we have Western blot analysis of connexin 43 knockout 293T cells, and that was done using Connexin 43 XP Rabbit mAb in the upper and GAPDH XP Rabbit mAb in the lower. Connexin 43 antibody specificity is demonstrated by the absence of a signal in connexin 43 knockout 293T cells.

References

Giepmans, B.N. et al. (2001) J Biol Chem 276, 8544-9. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 782