Difference between revisions of "Part:BBa K4886001"

Revision as of 04:55, 25 September 2023

Pthl-F/Xpk(BD)

It is the part which is responsible for the expression of F/Xpk from Clostridium acetobutylicum ATCC824 with Pthl promotor. It consists of Pthl sequence (BBa_K3443002), strong ribosomal binding site (RBS) sequence (BBa_K103015), F/Xpk sequence (BBa_K4119076) and terminator sequence (BBa_K3585002). F/Xpk is a gene that encodes phosphoketolase. Phosphoketolase is an enzyme with both the Fpk and Xpk activity. It catalyzes the conversion of fructose-6-phosphate (F6P) to erythrose-4-phosphate (E4P) and acetyl-phosphate (AcP), and the conversion of xylulose-5-phosphate (X5P) to glyceraldehydes-3-phosphate (G3P) and AcP.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1986
Illegal XbaI site found at 929
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1986
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1986
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1986
Illegal XbaI site found at 929
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1986
Illegal XbaI site found at 929
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4886001 short</partinfo>
+It is the part which is responsible for the expression of F/Xpk from Clostridium acetobutylicum ATCC824 with Pthl promotor. It consists of Pthl sequence (BBa_K3443002), strong ribosomal binding site (RBS) sequence (BBa_K103015), F/Xpk sequence (BBa_K4119076) and terminator sequence (BBa_K3585002).
+F/Xpk is a gene that encodes phosphoketolase. Phosphoketolase is an enzyme with both the Fpk and Xpk activity. It catalyzes the conversion of fructose-6-phosphate (F6P) to erythrose-4-phosphate (E4P) and acetyl-phosphate (AcP), and the conversion of xylulose-5-phosphate (X5P) to glyceraldehydes-3-phosphate (G3P) and AcP.
 <!-- Add more about the biology of this part here