Difference between revisions of "Part:BBa K4586016"

Revision as of 16:10, 24 September 2023

CMV

Part Description

CMV promoter is derived from human Cytomegalovirus, which belongs to Herpesvirus group. All family members share the ability to remain in latent stage in the human body. CMV is located upstream of immediate-early gene. However, CMV promoter is an example of widely used promoters and is present in mammalian expression vectors. The advantage of CMV is the high-level constitutive expression in mostly all human tissues. Due to its high transcription levels, it is a potent promoter that is employed to induce gene expression.

Usage

It is a promoter that controls the expression of CD63-L7Ae, the syn-notch receptor, and booster genes.

Literature Characterization

The study used Genomatix software for analysis of the CMV promoter (-550 to +48 relative to the transcription start site; TSS) for the presence of putative transcription factor regulatory elements (TFREs). They recognized one hundred and eight discrete TFREs in the CMV promoter for the presence of their cognate transcription factors (TFs) in HEK293 cells.

(A) THEY MADE RNA-seq analysis of the HEK293 cell transcriptome to assess the gene expression level of TFs. Points represent the expression level of each TF sampled at exponential and stationary phases of culture. They thought that genes with more than two transcripts per million (log2 TPM > 1) are actively transcribed genes. (B) They chose a CMV promoter sequence with 25 selected TFREs for in vitro analysis. The TSS is referred to with an arrow.

References

Johari, Y. B., Scarrott, J. M., Pohle, T. H., Liu, P., Mayer, A., Brown, A. J., & James, D. C. (2022). Engineering of the CMV promoter for controlled expression of recombinant genes in HEK293 cells. Biotechnology Journal, 17(8), 2200062. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 9: / Line 9: @@
 ==Usage==
 It is a promoter that controls the expression of CD63-L7Ae, the syn-notch receptor, and booster genes.
-<!-- Add more about the biology of this part here
+==Literature Characterization==
+The study used Genomatix software for analysis of the CMV promoter (-550 to +48 relative to the transcription start site; TSS) for the presence of putative transcription factor regulatory elements (TFREs). They recognized one hundred and eight discrete TFREs in the CMV promoter for the presence of their cognate transcription factors (TFs) in HEK293 cells.
+<html><div align="center"style="border:solid #17252A; width:50%;float:center;"><img style="                              	max-width:850px;
+							width:75%;
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+								position: relative;
+							top: 50%;
+							left: 35%;
+							transform: translate( -50%);
+							padding-bottom:25px;
+							padding-top:25px;
+							"src="https://static.igem.wiki/teams/4586/wiki/literature-characterisation-parts/cmv-promoter.png">
+<p class=MsoNormal align=center style='text-align:left;border:none;width:98% ;justify-content:center;'><span
+lang=EN style='font-size:11.0pt;line-height:115%'> (A) THEY MADE RNA-seq analysis of the HEK293 cell transcriptome to assess the gene expression level of TFs. Points represent the expression level of each TF sampled at exponential and stationary phases of culture. They thought that genes with more than two transcripts per million (log2 TPM > 1) are actively transcribed genes. (B) They chose a CMV promoter sequence with 25 selected TFREs for in vitro analysis. The TSS is referred to with an arrow.
+ </span></p></div></html>
+==References==
+Johari, Y. B., Scarrott, J. M., Pohle, T. H., Liu, P., Mayer, A., Brown, A. J., & James, D. C. (2022). Engineering of the CMV promoter for controlled expression of recombinant genes in HEK293 cells. Biotechnology Journal, 17(8), 2200062.<!-- Add more about the biology of this part here
 ===Usage and Biology===