Difference between revisions of "Part:BBa K4586015"

Figure 1: This figure illustrates the mechanism of loading our therapeutic agent in the form of mRNA selectively and efficiently into our engineered exosomes secreted form the MSCs,this loading is done through labeling the gene of interest with C\D box a hairpin structure IN THE 3` end this box have high affinity to the RNA binding protein L7Ae that is expressed on the internal surface of the engineered exosomes membrane conjugated to his tagged CD63 protein that is a natural highly expressed transmembrane protein within the exosomes.

Figure 2: This figure shows the design of the biological circuit expressing our loading system on the exosomes membrane ,this system consists of two main component ,First the RNA binding protein L7Ae conjugated to the second component, which is CD3 a transmembrane protein that is naturally expressed on the exosomes membrane.

By using Ribosomal Protein L7a (E109) Antibody, the study made Western blot analyses of extracts from various cell lines.

The study used the ribosomal protein L7a (E109) antibody, which appeared green, to make a confocal immunofluorescent analysis of COS cells. They labeled actin filaments with Alexa Fluor phalloidin 555, which appear red. DRAQ5 #4084 (fluorescent DNA dye) appeared as a blue pseudocolor. The antibody stains ribosomes at their biogenesis location in nucleoli.