Difference between revisions of "Part:BBa K4606001:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | We added ZsGreen to the transfection plasmid. In this way, when the plasmid was successfully transfected into the recipient cells, the ZsGreen was expressed, and the green fluorescence could be observed under the microscope. We can observe the success rate of plasmid transfection by this protein. | |
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===Source=== | ===Source=== | ||
| − | + | The fluorescent protein ZsGreen was selected from a combinatorial protein display library. | |
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===References=== | ===References=== | ||
| + | M'baye M, Hua G, Khan HA, Yang L. RNAi-mediated knockdown of INHBB increases apoptosis and inhibits steroidogenesis in mouse granulosa cells. J Reprod Dev. 2015;61(5):391-397. doi:10.1262/jrd.2014-158 | ||
Latest revision as of 01:51, 23 September 2023
ZsGreen
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We added ZsGreen to the transfection plasmid. In this way, when the plasmid was successfully transfected into the recipient cells, the ZsGreen was expressed, and the green fluorescence could be observed under the microscope. We can observe the success rate of plasmid transfection by this protein.
Source
The fluorescent protein ZsGreen was selected from a combinatorial protein display library.
References
M'baye M, Hua G, Khan HA, Yang L. RNAi-mediated knockdown of INHBB increases apoptosis and inhibits steroidogenesis in mouse granulosa cells. J Reprod Dev. 2015;61(5):391-397. doi:10.1262/jrd.2014-158