Difference between revisions of "Part:BBa K4586027"

 
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<partinfo>BBa_K4586027 short</partinfo>
 
<partinfo>BBa_K4586027 short</partinfo>
  
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This composite part designed to increase the default number of exosomes secreted from MSC.
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This composite part designed to increase the default number of exosomes secreted from MSC as shown in figure 1.
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<html><div align="center"style="border:solid #17252A; width:100%;float:center;"><img style="                              max-width:850px;
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width:100%;
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height:auto;
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position: relative;
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top: 50%;
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left: 45%;
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transform: translate( -50%);
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padding-bottom:25px;
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padding-top:25px;
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"src="https://static.igem.wiki/teams/4586/wiki/parts/booster-gene.png
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">
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<p class=MsoNormal align=center style='text-align:left;border:none;width:98% ;justify-content:center;'><span
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lang=EN style='font-size:11.0pt;line-height:115%'>Figure 1: This figure illustrates the design of our biological circuit coding for booster genes(SDC4,STEAP3 and NadB) and their role in increasing the synthetic capacity of MSCs to secrete exosomes that carry our therapeutic agent represented in Cas12k/gBAFF-R
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  </span></p></div></html>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 14:18, 22 September 2023


Booster genes (SDC4, STEAP3, NadB)

This composite part designed to increase the default number of exosomes secreted from MSC as shown in figure 1.

Figure 1: This figure illustrates the design of our biological circuit coding for booster genes(SDC4,STEAP3 and NadB) and their role in increasing the synthetic capacity of MSCs to secrete exosomes that carry our therapeutic agent represented in Cas12k/gBAFF-R

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 614
    Illegal BglII site found at 1152
    Illegal BglII site found at 2743
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3623
    Illegal AgeI site found at 4243
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2333