Difference between revisions of "Part:BBa K4586007"

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==Usage==
 
==Usage==
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In our project, this part is implemented in the DART V ADAR switch to be complementary to ACPA variable region mRNA with mismatched adenosine (A) group within the stop codon (UAG) that hinder the translation of the CRISPR-Cas system.  
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In our project, this part is implemented in the DART V ADAR switch to be complementary to ACPA variable region mRNA with mismatched adenosine (A) group within the stop codon (UAG) that hinder the translation of the CRISPR-Cas system as shown in figure 1.
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<html><div align="center"style="border:solid #17252A; width:100%;float:center;"><img style="                              max-width:850px;
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position: relative;
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top: 50%;
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"src="https://static.igem.wiki/teams/4586/wiki/parts/ms2-sensor-mcp-adar-7.png
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<p class=MsoNormal align=center style='text-align:left;border:none;width:98% ;justify-content:center;'><span
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lang=EN style='font-size:11.0pt;line-height:115%'>Figure 1: This figure illustrate the activity of our DART V ADAR tissue specific switch that is designed to be in the on state after recognition of the autoreactive B-cells,this recognition based on mismatched base editing in the level of transcribed RNA that is mediated through ADAR enzyme activity.  </span></p></div></html>
  
 
<!-- Add more about the biology of this part here
 
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Revision as of 12:44, 22 September 2023


Sensor of the DART V ADAR switch

Part Description

This part is our novel sensor, which is a single-stranded RNA that recognizes the specific complementary part and initiates the downstream transcription process to activate the CRISPR-Cas system.

Usage

In our project, this part is implemented in the DART V ADAR switch to be complementary to ACPA variable region mRNA with mismatched adenosine (A) group within the stop codon (UAG) that hinder the translation of the CRISPR-Cas system as shown in figure 1.

Figure 1: This figure illustrate the activity of our DART V ADAR tissue specific switch that is designed to be in the on state after recognition of the autoreactive B-cells,this recognition based on mismatched base editing in the level of transcribed RNA that is mediated through ADAR enzyme activity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]