Difference between revisions of "Part:BBa K4586002"

Revision as of 12:39, 22 September 2023

ZF21.16

Part Description

Zinc finger proteins are considered one of the most unique and huge collections of proteins that are variable in their biological function and structure. In our condition this zinc finger protein recognizes a specific DNA sequence thus ZF21.16 can be combined with any other protein as transcription factor to direct its activity toward the target sequences that are located near the gene of interest.

Usage

We used this part in our design to form the internal domain of our syn notch receptor (BBa_K4586022). This internal domain is released after receptor activation and guided by the ZF21.16 to the target promoter that regulates the levels of expression of our therapeutic agent as shown in figure 1.

Figure 1: This figure illustrates the design of our biological circuit expressing our therapeutic agent under the control of the VP64 transcription module that regulates the activity of the ZF21.16minCMV promoter.

Sequence and Features