Difference between revisions of "Part:BBa K283003"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K283003 short</partinfo>
 
<partinfo>BBa_K283003 short</partinfo>
  
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This part consists of a fusion protein invisine-RFP with a double terminator. There is no preceding rbs on the protein coding region.  
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This part generates the chez protein which is responsible for the dephosphorylation of cheY protein in bacteria flagella movement. A 6-histidine tail is added to the chez coding region. The pTet promoter can be repressed by the TetR repressor protein(C0040). It is constitutively on without the presence of TetR repressor.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 20:17, 16 October 2009

pTet-rbs-chez_histag-double terminator

This part generates the chez protein which is responsible for the dephosphorylation of cheY protein in bacteria flagella movement. A 6-histidine tail is added to the chez coding region. The pTet promoter can be repressed by the TetR repressor protein(C0040). It is constitutively on without the presence of TetR repressor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 726
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]