Difference between revisions of "Part:BBa K4579001"

 
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This is an inducible promoter part compatible with Golden Gate Assembly using the Bee Toolkit (BTK) syntax (Leonard et al., 2018). This part undergoes BsaI digestion to produce a linear part with overhangs characteristic of a Type 2 part in the BTK syntax. This part consists of the pTac promoter upstream of a hammerhead ribozyme, the latter of which helps insulate the transcript from sequence-specific interference effects. The pTac promoter includes the lac operator that may be bound by a LacI repressor protein, causing repression of transcription. LacI is removed from the operator sites when bound by the inducer molecule IPTG, allowing for the selective induction of transcription in cells containing both pTac and the lacI gene.
 
This is an inducible promoter part compatible with Golden Gate Assembly using the Bee Toolkit (BTK) syntax (Leonard et al., 2018). This part undergoes BsaI digestion to produce a linear part with overhangs characteristic of a Type 2 part in the BTK syntax. This part consists of the pTac promoter upstream of a hammerhead ribozyme, the latter of which helps insulate the transcript from sequence-specific interference effects. The pTac promoter includes the lac operator that may be bound by a LacI repressor protein, causing repression of transcription. LacI is removed from the operator sites when bound by the inducer molecule IPTG, allowing for the selective induction of transcription in cells containing both pTac and the lacI gene.
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References:
 
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1. Leonard et al. "Genetic Engineering of Bee Gut Microbiome Bacteria with a Toolkit for Modular Assembly of Broad-Host-Range Plasmids." ACS Synth Biology, 2018.
 
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2. Meyer et al. "Escherichia coli 'Marionette' strains with 12 highly optimized small-molecule sensors." Nature Chemical Biology, 2018.
 
  
 
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Revision as of 08:27, 22 September 2023


PTac promoter + RBS

This is an inducible promoter part compatible with Golden Gate Assembly using the Bee Toolkit (BTK) syntax (Leonard et al., 2018). This part undergoes BsaI digestion to produce a linear part with overhangs characteristic of a Type 2 part in the BTK syntax. This part consists of the pTac promoter upstream of a hammerhead ribozyme, the latter of which helps insulate the transcript from sequence-specific interference effects. The pTac promoter includes the lac operator that may be bound by a LacI repressor protein, causing repression of transcription. LacI is removed from the operator sites when bound by the inducer molecule IPTG, allowing for the selective induction of transcription in cells containing both pTac and the lacI gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]