Difference between revisions of "Part:BBa K4806224"

Revision as of 09:11, 21 September 2023

CYP9Q3 gene with mNeonGreen for Chlamydomonas reinhardtii (Phytobrick)

This composite part contains the AβSAP(i)-promotor (BBa_K4806013), the coding sequence of CYP9Q3 (BBa_K4806004), mNeonGreen (BBa_K4806006) and the tRPL23-terminator (BBa_K3002006)^*. This part is codon-optimized for Chlamydomonas reinhardtii and was built as part of the CYPurify Collection. This level 2 part leads to potential detoxification of specific chemicals (Ohkawa & Inui, 2015).

Construct

Fig.1 Construct design
This construct was designed using the modular cloning system (MoClo).

The resistance cassette for spectinomycin is already built in the level 2 vector pMBS807 we are using. The usage of this vector allows the direct assembly of level 0 parts to level 2 constructs, facilitating the cloning time (Niemeyer & Schroda, 2022).

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 1991
Illegal PstI site found at 2171
Illegal PstI site found at 2677
Illegal PstI site found at 3519
Illegal PstI site found at 3926
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 249
Illegal PstI site found at 1991
Illegal PstI site found at 2171
Illegal PstI site found at 2677
Illegal PstI site found at 3519
Illegal PstI site found at 3926
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 2533
Illegal BamHI site found at 3535
Illegal XhoI site found at 530
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 1991
Illegal PstI site found at 2171
Illegal PstI site found at 2677
Illegal PstI site found at 3519
Illegal PstI site found at 3926
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 1991
Illegal PstI site found at 2171
Illegal PstI site found at 2677
Illegal PstI site found at 3519
Illegal PstI site found at 3926
Illegal NgoMIV site found at 2948
Illegal NgoMIV site found at 4719
Illegal AgeI site found at 268
Illegal AgeI site found at 3864
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4806224 short</partinfo>
-a
-<!-- Add more about the biology of this part here
+<html>
-===Usage and Biology===
+<style>
+    .bild {max-width: 100% ; height: auto;}
+    .unterschrift {font-size: 11.5px;}
+</style>
-<!-- -->
-<span class='h3bb'>Sequence and Features</span>
-<partinfo>BBa_K4806224 SequenceAndFeatures</partinfo>
+<p>This composite part contains the AβSAP(i)-promotor (<a href=" https://parts.igem.org/Part:BBa_K4806013">BBa_K4806013</a>), the coding sequence of CYP9Q3 (<a href=" https://parts.igem.org/Part:BBa_K4806004">BBa_K4806004</a>), mNeonGreen (<a href=" https://parts.igem.org/Part:BBa_K4806006">BBa_K4806006</a>) and the tRPL23-terminator (<a href="https://parts.igem.org/Part:BBa_K3002006">BBa_K3002006</a>)<sup>*</sup>. This part is codon-optimized for <i>Chlamydomonas reinhardtii</i> and was built as part of the CYPurify Collection. This level 2 part leads to potential detoxification of specific chemicals (Ohkawa & Inui, 2015).</p>
+<br>
+<h2>Construct</h2>
+<p>
+  <img class="bild" src="https://static.igem.wiki/teams/4806/wiki/registry/level2/cyp9q3-mneon-construct.png">
+  <div class="unterschrift"><b>Fig.1 Construct design</b><br>
+   This construct was designed using the modular cloning system (MoClo).</div>
+</p>
+  <p>The resistance cassette for spectinomycin is already built in the level 2 vector pMBS807 we are using. The usage of this vector allows the direct assembly of level 0 parts to level 2 constructs, facilitating the cloning time (Niemeyer & Schroda, 2022). </p>
+<p><br></p>
+<h2>Sequence and Features</h2>
+</html>
+<partinfo>BBa_K4806224 SequenceAndFeatures</partinfo>
-<!-- Uncomment this to enable Functional Parameter display
-===Functional Parameters===
 <partinfo>BBa_K4806224 parameters</partinfo>
-<!-- -->
+<html>
+<h2>Results</h2>