Difference between revisions of "Part:BBa K4806213"

Revision as of 08:46, 21 September 2023

CYP3A4 gene with mNeonGreen for Chlamydomonas reinhardtii (Phytobrick)

This composite part contains the AβSAP(i)-promotor (BBa_K4806013), the coding sequence of the POR (BBa_K4806003), mNeonGreen (BBa_K4806006) and the tRPL23-terminator (BBa_K3002006)^*. This part is codon-optimized for Chlamydomonas reinhardtii and was built as part of the CYPurify Collection. This level 2 part leads to potential detoxification of specific chemicals (Ohkawa & Inui, 2015).

Construct

Fig.1 Construct design
This construct was designed using the modular cloning system (MoClo).

The resistance cassette for hygromycin is already built in the level 2 vector pMBS810 we are using. The usage of this vector allows the direct assembly of level 0 parts to level 2 constructs, facilitating the cloning time (Niemeyer & Schroda, 2022).

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 1353
Illegal PstI site found at 2613
Illegal PstI site found at 2673
Illegal PstI site found at 3332
Illegal PstI site found at 4144
Illegal PstI site found at 4551
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 249
Illegal NheI site found at 2538
Illegal PstI site found at 1353
Illegal PstI site found at 2613
Illegal PstI site found at 2673
Illegal PstI site found at 3332
Illegal PstI site found at 4144
Illegal PstI site found at 4551
Illegal NotI site found at 2978
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 4160
Illegal XhoI site found at 530
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 1353
Illegal PstI site found at 2613
Illegal PstI site found at 2673
Illegal PstI site found at 3332
Illegal PstI site found at 4144
Illegal PstI site found at 4551
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 1353
Illegal PstI site found at 2613
Illegal PstI site found at 2673
Illegal PstI site found at 3332
Illegal PstI site found at 4144
Illegal PstI site found at 4551
Illegal NgoMIV site found at 5344
Illegal AgeI site found at 268
Illegal AgeI site found at 4489
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
-<partinfo>BBa_K4806213 short</partinfo>
+<partinfo>BBa_K4806204 short</partinfo>
-a
-<!-- Add more about the biology of this part here
+<html>
-===Usage and Biology===
+<style>
+    .bild {max-width: 100% ; height: auto;}
+    .unterschrift {font-size: 11.5px;}
+</style>
-<!-- -->
-<span class='h3bb'>Sequence and Features</span>
-<partinfo>BBa_K4806213 SequenceAndFeatures</partinfo>
+<p>This composite part contains the AβSAP(i)-promotor (<a href=" https://parts.igem.org/Part:BBa_K4806013">BBa_K4806013</a>), the coding sequence of the POR (<a href=" https://parts.igem.org/Part:BBa_K4806003">BBa_K4806003</a>), mNeonGreen (<a href=" https://parts.igem.org/Part:BBa_K4806006">BBa_K4806006</a>) and the tRPL23-terminator (<a href="https://parts.igem.org/Part:BBa_K3002006">BBa_K3002006</a>)<sup>*</sup>. This part is codon-optimized for <i>Chlamydomonas reinhardtii</i> and was built as part of the CYPurify Collection. This level 2 part leads to potential detoxification of specific chemicals (Ohkawa & Inui, 2015).</p>
+<br>
+<h2>Construct</h2>
+<p>
+  <img class="bild" src="https://static.igem.wiki/teams/4806/wiki/registry/level2/por-mneongreen.png">
+  <div class="unterschrift"><b>Fig.1 Construct design</b><br>
+   This construct was designed using the modular cloning system (MoClo).</div>
+</p>
+  <p>The resistance cassette for hygromycin is already built in the level 2 vector pMBS810 we are using. The usage of this vector allows the direct assembly of level 0 parts to level 2 constructs, facilitating the cloning time (Niemeyer & Schroda, 2022). </p>
+<p><br></p>
+<h2>Sequence and Features</h2>
+</html>
+<partinfo>BBa_K4806213 SequenceAndFeatures</partinfo>
-<!-- Uncomment this to enable Functional Parameter display
-===Functional Parameters===
 <partinfo>BBa_K4806213 parameters</partinfo>
-<!-- -->
+<html>
+<h2>Results</h2>