Difference between revisions of "Part:BBa K4586007"
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==Part Description== | ==Part Description== | ||
| − | This part is a single-stranded RNA | + | This part is our novel sensor which is a single-stranded RNA that recognize the specific complementary part and initiate the downstream transcription process to activate the CRISPR-Cas system. |
| + | |||
==Usage== | ==Usage== | ||
In our project, this part is implemented in the DART V ADAR switch to be complementary to ACPA variable region mRNA with mismatched adenosine (A) group within the stop codon (UAG) that hinder the translation of the CRISPR-Cas system. | In our project, this part is implemented in the DART V ADAR switch to be complementary to ACPA variable region mRNA with mismatched adenosine (A) group within the stop codon (UAG) that hinder the translation of the CRISPR-Cas system. | ||
Revision as of 12:57, 20 September 2023
Sensor of the DART V ADAR switch
Part Description
This part is our novel sensor which is a single-stranded RNA that recognize the specific complementary part and initiate the downstream transcription process to activate the CRISPR-Cas system.
Usage
In our project, this part is implemented in the DART V ADAR switch to be complementary to ACPA variable region mRNA with mismatched adenosine (A) group within the stop codon (UAG) that hinder the translation of the CRISPR-Cas system.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]