Difference between revisions of "Part:BBa K4586005"

 
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<partinfo>BBa_K4586005 short</partinfo>
 
<partinfo>BBa_K4586005 short</partinfo>
  
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It is made up of two pieces. MCP, the ms2 coat protein, and ADAR, adenosine deaminases acting on double-stranded RNA, are the two. Through a process known as hybridization, ADAR can change mismatched adenosine (A) groups into inosine (I).
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==Part Description==
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This part codes for the catalytic portion of ADAR2 (adenosine deaminases acting on double-stranded RNA) that converts adenosines (A) to inosines (I) in both coding and non-coding RNA transcripts. Through a process known as hybridization or deamination, In addition to conjugating this part to MCP (MS2 coat protein), which has an affinity for the MS2 hairpin structure.
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==Usage==
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This part is implemented in our design to increase the effectivity and sensitivity of our therapeutic agent, as changing the status of the DART V ADAR switch from off to on is mediated through the ADAR enzyme by deamination of the mismatched adenosine group into an inosine group. Thus, the stop Codon UAG within the sensor of the switch will be converted into UIG, and the translation of the therapeutic agent will precede expressing Cas12k and MCP-ADAR2.  Thus, MCP-ADAR2 is creating a positive feedback loop that amplifies the signal transmitted from different cargo copies present within the target cell.
  
 
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Revision as of 09:37, 20 September 2023


MCP-ADAR2

Part Description

This part codes for the catalytic portion of ADAR2 (adenosine deaminases acting on double-stranded RNA) that converts adenosines (A) to inosines (I) in both coding and non-coding RNA transcripts. Through a process known as hybridization or deamination, In addition to conjugating this part to MCP (MS2 coat protein), which has an affinity for the MS2 hairpin structure.

Usage

This part is implemented in our design to increase the effectivity and sensitivity of our therapeutic agent, as changing the status of the DART V ADAR switch from off to on is mediated through the ADAR enzyme by deamination of the mismatched adenosine group into an inosine group. Thus, the stop Codon UAG within the sensor of the switch will be converted into UIG, and the translation of the therapeutic agent will precede expressing Cas12k and MCP-ADAR2. Thus, MCP-ADAR2 is creating a positive feedback loop that amplifies the signal transmitted from different cargo copies present within the target cell.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]