Difference between revisions of "Part:BBa K4719027"

 
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<partinfo>BBa_K4719027 short</partinfo>
 
<partinfo>BBa_K4719027 short</partinfo>
  
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We created a one-pot reaction method to produce a bacterial cellulose-chitosan copolymer. This was achieved by combining a construct that can synthesize a bacterial cellulose-chitin copolymer with a fused deacetylase enzyme. The deacetylase enzyme was linked to a signal peptide sequence, allowing for the secretion of the enzyme to target and modify N-Acetylglucosamine residues within the polymer.
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We created a one-pot reaction method to produce a bacterial cellulose-chitosan copolymer. This was achieved by combining a construct that can synthesize a bacterial cellulose-chitin copolymer with a deacetylase enzyme AnCDA. The deacetylase enzyme was linked to a signal peptide sequence, allowing for the secretion of the AnCDA to target and modify N-Acetylglucosamine residues within the polymer.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 06:19, 20 September 2023


AGM1-GFA1-GNA1-UAP1-AnCDA operon for one-step bacterial cellulose-chitosan synthesis

We created a one-pot reaction method to produce a bacterial cellulose-chitosan copolymer. This was achieved by combining a construct that can synthesize a bacterial cellulose-chitin copolymer with a deacetylase enzyme AnCDA. The deacetylase enzyme was linked to a signal peptide sequence, allowing for the secretion of the AnCDA to target and modify N-Acetylglucosamine residues within the polymer.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2076
    Illegal EcoRI site found at 2574
    Illegal EcoRI site found at 4000
    Illegal EcoRI site found at 5091
    Illegal XbaI site found at 3174
    Illegal SpeI site found at 37
    Illegal SpeI site found at 4867
    Illegal SpeI site found at 5755
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2076
    Illegal EcoRI site found at 2574
    Illegal EcoRI site found at 4000
    Illegal EcoRI site found at 5091
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 5908
    Illegal SpeI site found at 37
    Illegal SpeI site found at 4867
    Illegal SpeI site found at 5755
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2076
    Illegal EcoRI site found at 2574
    Illegal EcoRI site found at 4000
    Illegal EcoRI site found at 5091
    Illegal BglII site found at 1484
    Illegal BglII site found at 1583
    Illegal BglII site found at 3033
    Illegal BamHI site found at 4339
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2076
    Illegal EcoRI site found at 2574
    Illegal EcoRI site found at 4000
    Illegal EcoRI site found at 5091
    Illegal XbaI site found at 3174
    Illegal SpeI site found at 37
    Illegal SpeI site found at 4867
    Illegal SpeI site found at 5755
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2076
    Illegal EcoRI site found at 2574
    Illegal EcoRI site found at 4000
    Illegal EcoRI site found at 5091
    Illegal XbaI site found at 3174
    Illegal SpeI site found at 37
    Illegal SpeI site found at 4867
    Illegal SpeI site found at 5755
    Illegal NgoMIV site found at 5916
    Illegal NgoMIV site found at 5970
    Illegal NgoMIV site found at 6499
    Illegal AgeI site found at 5955
  • 1000
    COMPATIBLE WITH RFC[1000]