Difference between revisions of "Part:BBa K4891014:Design"

 
 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
Glucose facilitator encoded by Glk and Glf from Zymomonas mobilis is synthesized.
+
Glucokinase encoded by glk and glf gene from Zymomonas mobilis is codon-optimized and synthesized.
  
 
===References===
 
===References===

Latest revision as of 01:44, 20 September 2023


glk-glf


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 124
    Illegal PstI site found at 1136
    Illegal PstI site found at 1327
    Illegal PstI site found at 1900
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 124
    Illegal PstI site found at 1136
    Illegal PstI site found at 1327
    Illegal PstI site found at 1900
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 124
    Illegal PstI site found at 1136
    Illegal PstI site found at 1327
    Illegal PstI site found at 1900
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 124
    Illegal PstI site found at 1136
    Illegal PstI site found at 1327
    Illegal PstI site found at 1900
    Illegal AgeI site found at 421
    Illegal AgeI site found at 1312
    Illegal AgeI site found at 1468
    Illegal AgeI site found at 1480
    Illegal AgeI site found at 2191
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Glucose facilitator serves as an alternative for enhancing glucose transportation rate. Then, glucose could be transported into intracellular from extracellular.


Source

Glucokinase encoded by glk and glf gene from Zymomonas mobilis is codon-optimized and synthesized.

References