Difference between revisions of "Part:BBa K4891012:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | These recombinant plasmids serve as homologous fragments for knocking out the target genes by CRISPR-Cas9. | + | These recombinant plasmids pBAD33-ldhA/adhE/poxB/pta and pTargetF-sgRNAldhA/adhE/poxB/pta serve as homologous fragments and guide RNA for knocking out the target genes by CRISPR-Cas9, respectively. |
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===Source=== | ===Source=== | ||
− | + | ldhA (encoding lactate dehydrogenase), adhE (encoding alcohol dehydrogenase), poxB (encoding pyruvate dehydrogenase) and pta (encoding phosphate acetyltransferase) are obtained from the genome of E. coli MG1655. | |
===References=== | ===References=== |
Latest revision as of 01:22, 20 September 2023
poxB
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 651
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 651
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 651
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 651
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
These recombinant plasmids pBAD33-ldhA/adhE/poxB/pta and pTargetF-sgRNAldhA/adhE/poxB/pta serve as homologous fragments and guide RNA for knocking out the target genes by CRISPR-Cas9, respectively.
Source
ldhA (encoding lactate dehydrogenase), adhE (encoding alcohol dehydrogenase), poxB (encoding pyruvate dehydrogenase) and pta (encoding phosphate acetyltransferase) are obtained from the genome of E. coli MG1655.