Difference between revisions of "Part:BBa K4719005"

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Bacterial cellulose-chitin polymer was achieved by increasing the production of UDP-N-acetylglucosamine, which can be recognized as a viable substrate for cellulose synthase and incorporated in the bacterial cellulose polymer. We employed two strategies to produce this material. The first approach was to add N-acetylglucosamine into the growth medium [https://parts.igem.org/Part:BBa_K4719013 BBa_K4719013], and the second one was the production of N-acetylglucosamine by ''K. xylinus'' from simple sugars such as glucose, fructose, and saccharose in the growth medium [https://parts.igem.org/Part:BBa_K4719014 BBa_K4719014].
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Bacterial cellulose-chitin polymer was achieved by increasing the production of UDP-N-acetylglucosamine, which can be recognized as a viable substrate for cellulose synthase and incorporated in the bacterial cellulose polymer. We employed two strategies to produce this material. The first approach was to add N-acetylglucosamine into the growth medium [https://parts.igem.org/Part:BBa_K4719013 BBa_K4719013], and the second one was the production of N-acetylglucosamine by ''K. xylinus'' from other sugars such as glucose, fructose, and saccharose in the growth medium [https://parts.igem.org/Part:BBa_K4719014 BBa_K4719014].
  
 
===Usage and Biology===
 
===Usage and Biology===
  
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''GNA1'' is glucosamine 6-phosphate N-acetyltransferase. This enzyme catalyzes the transfer of an acetyl group from acetyl coenzyme A to glucosamine-6-phosphate to form N-acetylglucosamine-6-phosphate, which is an essential intermediate in UDP-GlcNAc biosynthesis. ''GNA1'' is a part in [https://parts.igem.org/Part:BBa_K4719014 BBa_K4719014].
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''GNA1'' is glucosamine 6-phosphate N-acetyltransferase. This enzyme catalyzes the transfer of an acetyl group from acetyl coenzyme A to glucosamine-6-phosphate to form N-acetylglucosamine-6-phosphate, which is an essential intermediate in UDP-GlcNAc biosynthesis[https://parts.igem.org/Part:BBa_K4719005#References (10)] . ''GNA1'' is a part in [https://parts.igem.org/Part:BBa_K4719014 BBa_K4719014].
  
 
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<partinfo>BBa_K4719005 parameters</partinfo>
 
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===References===
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1.Mio, T. et al. (1999) ‘Saccharomyces cerevisiae GNA1, an Essential Gene Encoding a Novel Acetyltransferase Involved in UDP-N-acetylglucosamine Synthesis’, Journal of Biological Chemistry, 274(1), pp. 424–429. doi:10.1074/jbc.274.1.424.

Revision as of 16:24, 19 September 2023


GNA1

Introduction

Vilnius Lithuania iGEM 2023 team's goal was to create a universal synthetic biology system in Komagataeibacter xylinus for in vivo bacterial cellulose polymer composition modification. Firstly, we chose to produce a cellulose-chitin polymer that would later be deacetylated, creating bacterial cellulose-chitosan. This polymer is an easily modifiable platform when compared to bacterial cellulose. The enhanced chemical reactivity of bacterial cellulose-chitosan polymer allows for specific functionalizations in the biomedicine field, such as scaffold design.

Bacterial cellulose-chitin polymer was achieved by increasing the production of UDP-N-acetylglucosamine, which can be recognized as a viable substrate for cellulose synthase and incorporated in the bacterial cellulose polymer. We employed two strategies to produce this material. The first approach was to add N-acetylglucosamine into the growth medium BBa_K4719013, and the second one was the production of N-acetylglucosamine by K. xylinus from other sugars such as glucose, fructose, and saccharose in the growth medium BBa_K4719014.

Usage and Biology

GNA1 is glucosamine 6-phosphate N-acetyltransferase. This enzyme catalyzes the transfer of an acetyl group from acetyl coenzyme A to glucosamine-6-phosphate to form N-acetylglucosamine-6-phosphate, which is an essential intermediate in UDP-GlcNAc biosynthesis(10) . GNA1 is a part in BBa_K4719014.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 118
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 118
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 118
    Illegal BamHI site found at 457
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 118
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 118
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1.Mio, T. et al. (1999) ‘Saccharomyces cerevisiae GNA1, an Essential Gene Encoding a Novel Acetyltransferase Involved in UDP-N-acetylglucosamine Synthesis’, Journal of Biological Chemistry, 274(1), pp. 424–429. doi:10.1074/jbc.274.1.424.