Difference between revisions of "Part:BBa K4891006:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Glucose facilitator serves as an alternative for enhancing glucose transportation rate. Then, glucose could be transported into intracellular from extracellular.
+
These recombinant plasmids pBAD33-aroK/aroL and pTargetF-sgRNAaroK/aroL serve as homologous fragments and guide RNA for knocking out the target genes by CRISPR-Cas9, respectively.
  
  
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===Source===
 
===Source===
  
−
Glucose facilitator encoded by Glk and Glf from Zymomonas mobilis is synthesized.
+
AroK and AroL (encoding shikimic acid kinase) are obtained from the genome of E. coli MG1655. In addition, recombinant plasmids pTargetF-sgRNAaroK/aroL initiate with pTargetF, which stores in our lab.
  
 
===References===
 
===References===

Revision as of 09:11, 19 September 2023


aroK


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 200


Design Notes

These recombinant plasmids pBAD33-aroK/aroL and pTargetF-sgRNAaroK/aroL serve as homologous fragments and guide RNA for knocking out the target genes by CRISPR-Cas9, respectively.


Source

AroK and AroL (encoding shikimic acid kinase) are obtained from the genome of E. coli MG1655. In addition, recombinant plasmids pTargetF-sgRNAaroK/aroL initiate with pTargetF, which stores in our lab.

References