Difference between revisions of "Part:BBa K203113:Design"

 
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<partinfo>BBa_K203113 short</partinfo>
 
<partinfo>BBa_K203113 short</partinfo>
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===Design Notes===
 
===Design Notes===
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To be updated
 
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CMV core promoter was amplified from pcDNA5/FRT (invitrogen) by using ATCGAAGCTTGGCGGTAGGCGTGTA as a forward and  CAGTGCTAGCatttcgataagccagtaagc as a reverse primer. PCR product and [[Part:BBa_K203112]] were cut with SpeI and HindIII, and ligated.
  
 
===Source===
 
===Source===
  
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To be updated
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For the CMV core promoter: pcDNA5/FRT (invitrogen)<br>
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For the JeT proximal promoter: Generated from [[Part:BBa_K203112]]
  
 
===References===
 
===References===

Latest revision as of 15:49, 16 October 2009

JeT proximal-CMV core; constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

CMV core promoter was amplified from pcDNA5/FRT (invitrogen) by using ATCGAAGCTTGGCGGTAGGCGTGTA as a forward and CAGTGCTAGCatttcgataagccagtaagc as a reverse primer. PCR product and Part:BBa_K203112 were cut with SpeI and HindIII, and ligated.

Source

For the CMV core promoter: pcDNA5/FRT (invitrogen)
For the JeT proximal promoter: Generated from Part:BBa_K203112

References