Difference between revisions of "Part:BBa K203112:Design"

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===References===
 
===References===
 +
[1] Tornoe, J. Generation of a synthetic mammalian promoter library by modification of sequences spacing transcription factor binding sites. Gene 297, 21-32 (2002). <br>
 +
[2] Stemmer, W.P.C. et al. Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides. Gene 164, 49-53 (1995).<br>
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[3] http://baderlab.bme.jhu.edu/gd/

Latest revision as of 14:52, 16 October 2009

JeT; constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence was adapted from [1] to include a HindIII site between core and proximal promoter (after base pair 66). Sequence was then synthesized by Assembly PCR [2] using Oligos designed using [3]:

JeT 01 GTACCAATTGGTGAGAATTCACTAGTGGGCGGAGTTA
JeT 02 CGCTGATTGGCTCCGCCCTAACTCCGCCCACTAGTGA
JeT 03 AGGGCGGAGCCAATCAGCGTGCGCCGTTCCGAAAGTT
JeT 04 CGCCCAGCCATAAAAGGCAACTTTCGGAACGGCGCAC
JeT 05 TGCCTTTTATGGCTGGGCGGAAGCTTAGAATGGGCGG
JeT 06 TATAATCATCGGCGTTCACCGCCCATTCTAAGCTTCC
JeT 07 GTGAACGCCGATGATTATATAAGGACGCGCCGGGTGT
JeT 08 GACGGAACTAGCTGTGCCACACCCGGCGCGTCCTTAT
JeT 09 TGGCACAGCTAGTTCCGTCGCAGCCGGGATTTGGGTCG
JeT 10 CTAGCACAAACAAGAACCGCGACCCAAATCCCGGCTGC
JeT 11 CGGTTCTTGTTTGTGCTAGCCTGCAGGTCAGAGATTGA
JeT 12 AGTCTGATCAATCTCTGACCTGCAGG

Source

Modified from [1]

References

[1] Tornoe, J. Generation of a synthetic mammalian promoter library by modification of sequences spacing transcription factor binding sites. Gene 297, 21-32 (2002).
[2] Stemmer, W.P.C. et al. Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides. Gene 164, 49-53 (1995).
[3] http://baderlab.bme.jhu.edu/gd/