Difference between revisions of "Part:BBa K4806001:Design"

(Replaced content with " __NOTOC__ <partinfo>BBa_K4806001 short</partinfo> <partinfo>BBa_K4806001 SequenceAndFeatures </partinfo> ===Design Notes=== This part has been codon optimized for Chl...")
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===Design Notes===
 
===Design Notes===
−
This part has been codon optimized for Chlamydomonas reinhardtii (https://www.kazusa.or.jp/codon/) <br>
+
This part has been codon optimized for Chlamydomonas reinhardtii (https://www.kazusa.or.jp/codon/) and it is compatible with Phytobricks /MoClo parts. <br>
−
We inserted a few mutations to eliminate the recognition sites for our type IIS restriction enzymes BbsI and BsaI.
+
We had to insert one mutation
  
  

Revision as of 20:20, 14 September 2023


CYP2D6 gene for Chlamydomonas reinhardtii (Phytobrick)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 356
    Illegal PstI site found at 1712
    Illegal PstI site found at 2145
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 356
    Illegal PstI site found at 1712
    Illegal PstI site found at 2145
    Illegal NotI site found at 862
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 356
    Illegal PstI site found at 1712
    Illegal PstI site found at 2145
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 356
    Illegal PstI site found at 1712
    Illegal PstI site found at 2145
    Illegal NgoMIV site found at 999
    Illegal NgoMIV site found at 1348
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has been codon optimized for Chlamydomonas reinhardtii (https://www.kazusa.or.jp/codon/) and it is compatible with Phytobricks /MoClo parts.
We had to insert one mutation


Source

https://www.uniprot.org/uniprotkb/P10635/entry

References