Difference between revisions of "Part:BBa K4606004:Design"
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<partinfo>BBa_K4606004 short</partinfo>
 
<partinfo>BBa_K4606004 short</partinfo>
  
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<partinfo>BBa_K4606004 SequenceAndFeatures</partinfo>
 
 
For detailed design information, you can look up the registry of (BBa_K 4606003), the device for gene segment of SMAD4.
 
For detailed design information, you can look up the registry of (BBa_K 4606003), the device for gene segment of SMAD4.
 
According to the base sequence information of SMAD4 in the database, we mutated the SMAD4 gene in a region that complementary binds to miR-3074-5p. By inserting its 3’UTR region (414bp) behind the luciferase gene in the vector, we could determine the expression level of this gene segment.
 
According to the base sequence information of SMAD4 in the database, we mutated the SMAD4 gene in a region that complementary binds to miR-3074-5p. By inserting its 3’UTR region (414bp) behind the luciferase gene in the vector, we could determine the expression level of this gene segment.
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<partinfo>BBa_K4606004 SequenceAndFeatures</partinfo>
  
  

Revision as of 14:06, 14 September 2023


SMAD4(mutant type)

For detailed design information, you can look up the registry of (BBa_K 4606003), the device for gene segment of SMAD4. According to the base sequence information of SMAD4 in the database, we mutated the SMAD4 gene in a region that complementary binds to miR-3074-5p. By inserting its 3’UTR region (414bp) behind the luciferase gene in the vector, we could determine the expression level of this gene segment.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

AAA


Source

AAA

References