Difference between revisions of "Part:BBa K180009:Design"

 
(→‎Design Notes)
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__NOTOC__
 
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<partinfo>BBa_K180009 short</partinfo>
 
<partinfo>BBa_K180009 short</partinfo>
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===Design Notes===
 
===Design Notes===
 
PstI site within the mBanana sequence - has to be assembled using BioBrick assembly in such an order that any successive digestions will not require PstI, otherwise the mCherry sequence will be cut as well as the site of interest. Alternatively, the assembly can be performed using non-standard BioBrick assembly.
 
PstI site within the mBanana sequence - has to be assembled using BioBrick assembly in such an order that any successive digestions will not require PstI, otherwise the mCherry sequence will be cut as well as the site of interest. Alternatively, the assembly can be performed using non-standard BioBrick assembly.
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Part cannot be submitted to registry as the rights are owned by Clontech. The mCherry sequence is however commercially available from Clontech in the pmBanana vector.
 
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'''Part cannot be submitted to registry as the rights are owned by Clontech. The mBanana sequence is however commercially available from Clontech in the pmBanana vector.'''
  
 
===Source===
 
===Source===

Revision as of 14:13, 16 October 2009

mBanana (rights owned by Clontech)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 352
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 352
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 352
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 352
    Illegal NgoMIV site found at 598
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 541


Design Notes

PstI site within the mBanana sequence - has to be assembled using BioBrick assembly in such an order that any successive digestions will not require PstI, otherwise the mCherry sequence will be cut as well as the site of interest. Alternatively, the assembly can be performed using non-standard BioBrick assembly.

Part cannot be submitted to registry as the rights are owned by Clontech. The mBanana sequence is however commercially available from Clontech in the pmBanana vector.

Source

pmBanana vector, Clontech

References