Difference between revisions of "Part:BBa K4990007"

(→‎What is it?)
Line 38: Line 38:
 
===What is it?===
 
===What is it?===
 
Here is the structure of HlpA monomer.
 
Here is the structure of HlpA monomer.
−
https://static.igem.wiki/teams/4990/wiki/registry/title7.png
+
<html>
 +
<img src="https://static.igem.wiki/teams/4990/wiki/registry/structure.png" width="450" >
 +
</html>
 
Here is the structure of HlpA homodimer.
 
Here is the structure of HlpA homodimer.
−
 
+
<html>
−
Two helical segments from each monomeric subunit constitute an �-helical ‘body’ with two protruding �-ribbon ‘arms’ , which extend to bind the DNA helix. These DNA-binding �-ribbons are largely disordered in the absence of DNA
+
<img src="https://static.igem.wiki/teams/4990/wiki/registry/structure2.png" width="450" >
 +
</html>
 +
Two helical segments from each monomeric subunit constitute an α-helical ‘body’ with two protruding β-ribbon ‘arms’ , which extend to bind the DNA helix. These DNA-binding β-ribbons are largely disordered in the absence of DNA
  
 
===Referrence===
 
===Referrence===

Revision as of 13:55, 13 September 2023


HlpA

Usage in short

You can use it to target coloretal cancer!

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 231
  • 1000
    COMPATIBLE WITH RFC[1000]

What you need to know!

2006年Tjalsma通过Highly accurate tandem MS方法，找到了一种蛋白Histone-like protein A(HlpA),发现它联系Streptococcus bovis和结直肠癌的桥梁，并推测S.bovis正是通过HlpA与结直肠癌细胞表面的heparan sulfate-proteoglycans(HSPG)结合，介导的S.bovis定植于结直肠癌[1]。

2009年Boleij证明了Streptococcus gallolyticus通过HlpA于结直肠癌细胞表面的HSPG结合[2]。

2016年O'Neil得到了Hlp的晶体结构，指示Hlp为类似蟹钳的结构，钳子部分的碱性氨基酸可以与DNA结合，同时也可以与肝素结合[3]。

2018年Chun Loong Ho利用HlpA与HSPG的结合，构建了一种可以靶向结直肠癌的工程大肠杆菌，拉开了HlpA靶向系统的序幕[4]。

2022年iGEM22_LZU-CHINA 团队，通过合成生物学构建了靶向结直肠癌的大肠杆菌，方法为HlpA靶向肿瘤细胞表面的HSPG[5]。

2023年Tang构建了利用HlpA靶向，Azurin杀伤的工程益生菌，并在结直肠癌小鼠中展现出不错的疗效[6]。

control
Figure A:Crystal structure of HlpA(O'Neil 2016) Figure B:Engineered EcN treat CRC(Chun 2018)

What is it?

Here is the structure of HlpA monomer. Here is the structure of HlpA homodimer. Two helical segments from each monomeric subunit constitute an α-helical ‘body’ with two protruding β-ribbon ‘arms’ , which extend to bind the DNA helix. These DNA-binding β-ribbons are largely disordered in the absence of DNA

Referrence

[1]Tjalsma H, Schöller‐Guinard M, Lasonder E, et al. Profiling the humoral immune response in colon cancer patients: diagnostic antigens from Streptococcus bovis[J]. International journal of cancer, 2006, 119(9): 2127-2135.

[2]Boleij A, Schaeps R M J, de Kleijn S, et al. Surface-exposed histone-like protein a modulates adherence of Streptococcus gallolyticus to colon adenocarcinoma cells[J]. Infection and immunity, 2009, 77(12): 5519-5527.

[3]O'Neil P, Lovell S, Mehzabeen N, et al. Crystal structure of histone-like protein from Streptococcus mutans refined to 1.9 Å resolution[J]. Acta Crystallographica Section F: Structural Biology Communications, 2016, 72(4): 257-262.

[4]Ho C L, Tan H Q, Chua K J, et al. Engineered commensal microbes for diet-mediated colorectal-cancer chemoprevention[J]. Nature biomedical engineering, 2018, 2(1): 27-37.

[5]https://2022.igem.wiki/lzu-china/

[6]