Difference between revisions of "Part:BBa K4806001"
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     Here are the links to the built constructs: and transformed these into Chlamydomonas reinhardtii.<br>
 
     Here are the links to the built constructs: and transformed these into Chlamydomonas reinhardtii.<br>
 
<ul>
 
<ul>
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<li>1. CYP2D6 gene with HA-tag for Chlamydomonas reinhardtii (Phytobrick) ()</li>
+
<li>1. CYP2D6 gene with HA-tag for Chlamydomonas reinhardtii (Phytobrick) (<a href=" https://parts.igem.org/Part:BBa_K4806205">BBa_K4806205</a>)</li>
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<li>2. CYP2D6 gene with mStop for Chlamydomonas reinhardtii (Phytobrick) ()</li>
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<li>2. CYP2D6 gene with mStop for Chlamydomonas reinhardtii (Phytobrick) (<a href=" https://parts.igem.org/Part:BBa_K4806207">BBa_K4806207</a>)</li>
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<li>3. CYP2D6 gene with FLAG-tag for Chlamydomonas reinhardtii (Phytobrick) ()</li>
+
<li>3. CYP2D6 gene with FLAG-tag for Chlamydomonas reinhardtii (Phytobrick) (<a href=" https://parts.igem.org/Part:BBa_K4806206">BBa_K4806206</a>)</li>
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<li>4. CYP2D6 gene for expression in the chloroplast for Chlamydomonas reinhardtii (Phytobrick) ()</li>
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<li>4. CYP2D6 gene for expression in the chloroplast for Chlamydomonas reinhardtii (Phytobrick) (<a href=" https://parts.igem.org/Part:BBa_K4806208">BBa_K4806208</a>)</li>
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<li>5. CYP2D6 tandem for expression together with the POR for Chlamydomonas reinhardtii (Phytobrick) ()</li>
+
<li>5. CYP2D6 tandem for expression together with the POR for Chlamydomonas reinhardtii (Phytobrick) (<a href=" https://parts.igem.org/Part:BBa_K4806215">BBa_K4806215</a>)</li>
 
</ul>
 
</ul>
 
</p>
 
</p>
  
 
</html>
 
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Revision as of 10:42, 13 September 2023

CYP2D6 gene for Chlamydomonas reinhardtii (Phytobrick)

This basic part contains the coding sequence of CYP2D6 (B3-B4). This part is codon-optimized for Chlamydomonas reinhardtii and was built as part of the CYPurify Collection. In combination with a promoter like AβSAP(i) (BBa_K4806013) and a terminator like tRPL23 (BBa_K3002006), this level 0 part leads to expression and potential detoxification of specific chemicals (Ohkawa & Inui, 2015). To detect the target protein a tag like FLAG-tag (BBa_K4806012) is recommended.


Constructs

Fig.1 Construct design
We designed 5 level 2 parts containing CYP2D6 using the modular cloning system (MoClo).


Here are the links to the built constructs: and transformed these into Chlamydomonas reinhardtii.

  • 1. CYP2D6 gene with HA-tag for Chlamydomonas reinhardtii (Phytobrick) (BBa_K4806205)
  • 2. CYP2D6 gene with mStop for Chlamydomonas reinhardtii (Phytobrick) (BBa_K4806207)
  • 3. CYP2D6 gene with FLAG-tag for Chlamydomonas reinhardtii (Phytobrick) (BBa_K4806206)
  • 4. CYP2D6 gene for expression in the chloroplast for Chlamydomonas reinhardtii (Phytobrick) (BBa_K4806208)
  • 5. CYP2D6 tandem for expression together with the POR for Chlamydomonas reinhardtii (Phytobrick) (BBa_K4806215)