Difference between revisions of "Part:BBa K4656001"
(→‎Usage and Biology)
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===Usage and Biology===
 
===Usage and Biology===
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Our project uses the introduction of Tdc sequences and inducing their expression to produce serotonin to test their efficacy in treating constipation.
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In our project, tdc and tph together catalyze the generation of 5-HT，when sensing a decrease in butyrate concentration.
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===Sequence and Features===
 
===Sequence and Features===

Revision as of 15:25, 9 September 2023


TDC

Tdc can remove the carboxyl group from 5-hydroxytryptophan (5-HTP) and convert it into serotonin (5-hydroxytryptophan, 5-HT) molecules. Tryptophan decarboxylase exists widely in animals and plants, especially in the central nervous system and gastrointestinal tract. 5-HTP is a precursor substance of neurotransmitter, while serotonin is an important neurotransmitter, similar to intestinal motor in terms of intestinal physiological function, and plays an important role in alleviating chronic constipation.

Usage and Biology

In our project, tdc and tph together catalyze the generation of 5-HT，when sensing a decrease in butyrate concentration.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1248
    Illegal BamHI site found at 732
    Illegal BamHI site found at 834
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 340
    Illegal NgoMIV site found at 775
    Illegal NgoMIV site found at 1330
    Illegal AgeI site found at 478
    Illegal AgeI site found at 1402
  • 1000
    COMPATIBLE WITH RFC[1000]