Difference between revisions of "Part:BBa K4719020"

 
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<partinfo>BBa_K4719020 short</partinfo>
 
<partinfo>BBa_K4719020 short</partinfo>
  
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We fused a linker connected to a cellulose binding domain to the N-terminus of deacetylase ArCE4A to ensure a higher degree of deacetylation. For protein purification 6x his-tag was added to the N-terminus of cellulose binding domain.
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We fused a cellulose binding domain connected to a linker to the N-terminus of deacetylase ArCE4A to ensure a higher degree of deacetylation. For protein purification 6x his-tag was added to the N-terminus of cellulose binding domain.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 08:06, 7 September 2023


CBD-ProThr box-ArCE4A chitin deacetylase and cellulose binding domain fusion protein

We fused a cellulose binding domain connected to a linker to the N-terminus of deacetylase ArCE4A to ensure a higher degree of deacetylation. For protein purification 6x his-tag was added to the N-terminus of cellulose binding domain.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 432
    Illegal NgoMIV site found at 961
    Illegal AgeI site found at 417
  • 1000
    COMPATIBLE WITH RFC[1000]