Difference between revisions of "Part:BBa K4607008"

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The biobrick consists of the polycationic nonapeptide (PCNP) which is capable to destabilize the lipopolysaccharide binds on Gram-negative baterium. The principle about the PCNP is related to the ionic interactions between the phosphate groups, divalent cations, and hyrdophobic lipids' stacking, where the PCNP acts as a destabilizing. This part has been fused with endolysins in order to increase their capability to lyse Gram-negative bacterium, demonstrating that the addition of the PCNP allows the endolysin introduction into the bacteria cell membrane. It part have been evaluated in <i>Escherichia coli</i>. The peptide has a lenght of nine amino acids.  
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The biobrick consists of the polycationic nonapeptide (PCNP) which is capable to destabilize the lipopolysaccharide binds on Gram-negative baterium. The principle about the PCNP is related to the ionic interactions between the phosphate groups, divalent cations, and hyrdophobic lipids' stacking, where the PCNP acts as a destabilizing. This part has been fused with endolysins in order to increase their capability to lyse Gram-negative bacterium, demonstrating that the addition of the PCNP allows the endolysin introduction into the bacteria cell membrane. It part have been evaluated in <i>Escherichia coli</i>. The peptide has a lenght of nine amino acids [1].  
  
  

Revision as of 19:28, 6 September 2023


PCNP

Description


The biobrick consists of the polycationic nonapeptide (PCNP) which is capable to destabilize the lipopolysaccharide binds on Gram-negative baterium. The principle about the PCNP is related to the ionic interactions between the phosphate groups, divalent cations, and hyrdophobic lipids' stacking, where the PCNP acts as a destabilizing. This part has been fused with endolysins in order to increase their capability to lyse Gram-negative bacterium, demonstrating that the addition of the PCNP allows the endolysin introduction into the bacteria cell membrane. It part have been evaluated in Escherichia coli. The peptide has a lenght of nine amino acids [1].


imagen
Figure 1. PCNP diagram.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

References

[1] Briers, Y., Walmagh, M., Van Puyenbroeck, V., Cornelissen, A., Cenens, W., Aertsen, A., Oliveira, H., Azeredo, J., Verween, G., Pirnay, J.-P., Miller, S., Volckaert, G., & Lavigne, R. (2014). Engineered endolysin-based “Artilysins” to combat multidrug-resistant gram-negative pathogens. MBio, 5(4), e01379-01314. https://doi.org/10.1128/mBio.01379-14 ‌

===References===