Difference between revisions of "Part:BBa K4607008"
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K4607008 parameters</partinfo>
 
<partinfo>BBa_K4607008 parameters</partinfo>
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===Plasmid Construction and Analysis===
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===Golden Gate Assembly and identification===
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===Protein Induction Kinetic===
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===Massive Protein Induction===

Revision as of 04:40, 6 September 2023


PCNP

Description


The biobrick consists of the polycationic nonapeptide (PCNP) which is capable to destabilize the lipopolysaccharide binds on Gram-negative baterium. The principle about the PCNP is related to the ionic interactions between the phosphate groups, divalent cations, and hyrdophobic lipid's stacking, where the PCNP acts as a destabilizing.

The peptide has a lenght of nine amino acids.



imagen
Figure 1. PCNP diagram.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology