Difference between revisions of "Part:BBa K4727000"

Revision as of 19:33, 23 August 2023

A. baumannii & K. pneumoniae standard backbone

This plasmid backbone was developed by Wang et al [1], it is provided of two ORIs: the first one a pMB1/pUC high copy number ORI for E. coli, the second one is capable o maintaining the plasmid both in Acinetobacter baumannii and Klebsiella pneumoniae

This backbone has been made standard RFC[10] compatible and provided with the RFC[10] prefix and suffix. Flanked by prefix and suffix is located part BBa_J23119 that has been demonstrated able to promote mRNA synthesis in K. pneumoniae and A. baumannii

The plasmid brings AmpR as a selection marker

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal prefix found at 3664
Illegal suffix found at 3721
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3664
Illegal NheI site found at 969
Illegal NheI site found at 3692
Illegal NheI site found at 3715
Illegal SpeI site found at 3722
Illegal PstI site found at 3736
Illegal NotI site found at 3670
Illegal NotI site found at 3729
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3664
Illegal BglII site found at 1162
Illegal XhoI site found at 1775
Illegal XhoI site found at 3780
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found at 3664
Illegal suffix found at 3722
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 3664
Plasmid lacks a suffix.
Illegal XbaI site found at 3679
Illegal SpeI site found at 3722
Illegal PstI site found at 3736
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal SapI site found at 4008

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