Difference between revisions of "Part:BBa K4905013"

Revision as of 09:15, 16 August 2023

Elastin-Like Polypeptide Triblock with FKBP12 and FRB

This part is made up of parts BBa_J18926, BBa_K4905001, BBa_K4905002, BBa_K3610022. It was used by the TU Eindhoven 2023 team to form a hydrogel outside as well as inside e.coli BL21 cells.

The construct consists of Elastin-like Polypeptides (ELPs) and rapamycin binding domains. Rapamycin is a known molecular glue that can be used to link FRB and FKBP12 protein domains together. ELPs have hydrophilic and hydrophobic domains that exhibit reversible phase separation behavior that is temperature dependent. This allows them to be used in the formation of a hydrogel.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 2177
Illegal XbaI site found at 294
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 2177
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 2177
Illegal BglII site found at 225
Illegal XhoI site found at 2194
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 2177
Illegal XbaI site found at 294
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 2177
Illegal XbaI site found at 294
Illegal NgoMIV site found at 351
Illegal NgoMIV site found at 531
Illegal NgoMIV site found at 621
Illegal NgoMIV site found at 801
Illegal NgoMIV site found at 3123
Illegal NgoMIV site found at 3300
Illegal NgoMIV site found at 3390
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4905013 short</partinfo>
-This part is made up of parts BBa_J18926, BBa_K4905001, BBa_K4905002, BBa_K3610022. It was used by the Eindhoven 2023 team to form a hydrogel outside as well as inside e.coli BL21 cells.
+This part is made up of parts [https://parts.igem.org/Part:BBa_J18926 BBa_J18926], [https://parts.igem.org/Part:BBa_K4905001 BBa_K4905001], [https://parts.igem.org/Part:BBa_K4905002 BBa_K4905002], [https://parts.igem.org/Part:BBa_K3610022 BBa_K3610022]. It was used by the TU Eindhoven 2023 team to form a hydrogel outside as well as inside e.coli BL21 cells.
-The construct consists of Elastin-like Polypeptides (ELPs) and rapamyacin binding domains. Rapamyacin is a known molecular glue that can be used to link FRB and FKBP12 protein domains together. ELPs have hydrophilic and hydrophobic domains that exhibit reversible phase separation behavior that is temperature dependent. This allows them to be used in the formation of a hydrogel.
+The construct consists of Elastin-like Polypeptides (ELPs) and rapamycin binding domains. Rapamycin is a known molecular glue that can be used to link FRB and FKBP12 protein domains together. ELPs have hydrophilic and hydrophobic domains that exhibit reversible phase separation behavior that is temperature dependent. This allows them to be used in the formation of a hydrogel.