Difference between revisions of "Part:BBa K4905007"

Revision as of 09:06, 16 August 2023

Elastin-Like Polypeptide Triblock with Leucine Zippers

This part is made up of parts BBa_K4905004, BBa_K4905005, BBa_K4905001 and BBa_K4905002. It was used by the TU Eindhoven 2023 team to form a hydrogel outside as well as inside e.coli BL21 cells.

The construct consists of Elastin-like Polypeptides (ELPs) and two leucine zippers that have affinity for each other. ELPs have hydrophilic and hydrophobic domains that exhibit reversible phase separation behavior that is temperature dependent. This allows them to be used in the formation of a reversible hydrogel.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1982
Illegal XbaI site found at 99
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1982
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1982
Illegal XhoI site found at 1999
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1982
Illegal XbaI site found at 99
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1982
Illegal XbaI site found at 99
Illegal NgoMIV site found at 156
Illegal NgoMIV site found at 336
Illegal NgoMIV site found at 426
Illegal NgoMIV site found at 606
Illegal NgoMIV site found at 2928
Illegal NgoMIV site found at 3105
Illegal NgoMIV site found at 3195
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4905007 short</partinfo>
-This part is made up of parts BBa_K4905004, BBa_K4905005, BBa_K4905001 and BBa_K4905002 . It was used by the Eindhoven 2023 team to form a hydrogel outside as well as inside e.coli BL21 cells.
+This part is made up of parts [https://parts.igem.org/Part:BBa_K4905004 BBa_K4905004], [https://parts.igem.org/Part:BBa_K4905005 BBa_K4905005], [https://parts.igem.org/Part:BBa_K4905001 BBa_K4905001] and [https://parts.igem.org/Part:BBa_K4905002 BBa_K4905002]. It was used by the TU Eindhoven 2023 team to form a hydrogel outside as well as inside e.coli BL21 cells.
 The construct consists of Elastin-like Polypeptides (ELPs) and two leucine zippers that have affinity for each other. ELPs have hydrophilic and hydrophobic domains that exhibit reversible phase separation behavior that is temperature dependent. This allows them to be used in the formation of a reversible hydrogel.