Difference between revisions of "Part:BBa K203109"

 
 
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<partinfo>BBa_K203109 short</partinfo>
 
<partinfo>BBa_K203109 short</partinfo>
  
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A constitutive promoter from the Heidelberg 2009 library of promoters for use in mammalian cells.
  
 
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K203109 parameters</partinfo>
 
<partinfo>BBa_K203109 parameters</partinfo>
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We generated a library of constitutive promoters by [http://2009.igem.org/Team:Heidelberg/Project_Synthetic_promoters RA-PCR]. We characterized those promoters by Flow cytometry.
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[[Image:HD09_constitutive.png]]
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This promoter corresponds to clone S4. We confirmed the initial measurement by an independet technique (image analysis). Its expression strength is approx 2,5 [http://2009.igem.org/Team:Heidelberg/Project_Measurement REU].
 
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Latest revision as of 09:17, 16 October 2009

Constitutive promoter; 2.5 REU

A constitutive promoter from the Heidelberg 2009 library of promoters for use in mammalian cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

We generated a library of constitutive promoters by [http://2009.igem.org/Team:Heidelberg/Project_Synthetic_promoters RA-PCR]. We characterized those promoters by Flow cytometry.

HD09 constitutive.png

This promoter corresponds to clone S4. We confirmed the initial measurement by an independet technique (image analysis). Its expression strength is approx 2,5 [http://2009.igem.org/Team:Heidelberg/Project_Measurement REU].