Difference between revisions of "Part:BBa K203111"
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K203110 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K203111 SequenceAndFeatures</partinfo>
  
 
===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K203110 parameters</partinfo>
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<partinfo>BBa_K203111 parameters</partinfo>
  
 
We generated a library of constitutive promoters by [http://2009.igem.org/Team:Heidelberg/Project_Synthetic_promoters RA-PCR]. We characterized those promoters by Flow cytometry.
 
We generated a library of constitutive promoters by [http://2009.igem.org/Team:Heidelberg/Project_Synthetic_promoters RA-PCR]. We characterized those promoters by Flow cytometry.

Revision as of 09:05, 16 October 2009

Constitutive promoter; 2 REU

A constitutive promoter from the Heidelberg 2009 library of promoters for use in mammalian cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Functional Parameters

We generated a library of constitutive promoters by [http://2009.igem.org/Team:Heidelberg/Project_Synthetic_promoters RA-PCR]. We characterized those promoters by Flow cytometry.

HD09 constitutive.png

This promoter corresponds to clone S10. We confirmed the initial measurement by an independet technique (image analysis). Its expression strength is approx. 2 [http://2009.igem.org/Team:Heidelberg/Project_Measurement REU].