Difference between revisions of "Part:BBa K4790048"
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<partinfo>BBa_K4790048 short</partinfo> | <partinfo>BBa_K4790048 short</partinfo> | ||
| − | The primer was designed to perform site-directed mutation which was defined as a forward primer. It can mutate base sequence which is on code 59 and coding the 81st amino acid from aspartic acid to histidine. According to Codon preference in E.coli, CAT has the highest possibility coding | + | The primer was designed to perform site-directed mutation which was defined as a forward primer. It can mutate base sequence which is on code 59 and coding the 81st amino acid from aspartic acid to histidine. According to Codon preference in E.coli, CAT has the highest possibility coding histidine. |
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Revision as of 07:41, 14 August 2023
ASR1-N81H-F
The primer was designed to perform site-directed mutation which was defined as a forward primer. It can mutate base sequence which is on code 59 and coding the 81st amino acid from aspartic acid to histidine. According to Codon preference in E.coli, CAT has the highest possibility coding histidine.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 5
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 5
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 5
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 5
- 1000COMPATIBLE WITH RFC[1000]