Difference between revisions of "Part:BBa K4765014"
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− | + | ==Introduction== | |
− | completes the production of palythines from disubstituted MAAs. | + | Nonheme iron-(II)- and 2oxoglutarate-dependent (Fe/2OG) oxygenase gene MysH completes the production of palythines from disubstituted MAAs<ref>Chen, M., Rubin, G. M., Jiang, G., Raad, Z., & Ding, Y. (2021). Biosynthesis and heterologous production of mycosporine-like amino acid palythines. ''The Journal of Organic Chemistry, 86''(16), 11160–11168.</ref>. |
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+ | ==Usage and Biology== | ||
+ | We performed codon optimization on [https://parts.igem.org/Part:BBa_K4273014 BBa_K4273014] (NlmysH) specifically for the ''Escherichia coli'' K12 strain, resulting in the creation of this part. The enzyme MysH catalyzes the final reaction involved in the biosynthesis of Mycosporine-like amino acids (MAAs) within ''E. coli''. | ||
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<partinfo>BBa_K4765008 parameters</partinfo> | <partinfo>BBa_K4765008 parameters</partinfo> | ||
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+ | ==References== | ||
+ | <references /> |
Revision as of 07:54, 11 August 2023
INPNC
Introduction
Nonheme iron-(II)- and 2oxoglutarate-dependent (Fe/2OG) oxygenase gene MysH completes the production of palythines from disubstituted MAAs[1].
Usage and Biology
We performed codon optimization on BBa_K4273014 (NlmysH) specifically for the Escherichia coli K12 strain, resulting in the creation of this part. The enzyme MysH catalyzes the final reaction involved in the biosynthesis of Mycosporine-like amino acids (MAAs) within E. coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 481
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 330
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 72
Illegal NgoMIV site found at 405
Illegal AgeI site found at 429 - 1000COMPATIBLE WITH RFC[1000]
References
- ↑ Chen, M., Rubin, G. M., Jiang, G., Raad, Z., & Ding, Y. (2021). Biosynthesis and heterologous production of mycosporine-like amino acid palythines. The Journal of Organic Chemistry, 86(16), 11160–11168.