Difference between revisions of "Part:BBa K4656008:Design"
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The pchA and PpchA is from its organism, the enterohemorrhagic Escherichia coli strain O157:H7 (strain: Sakai, substrain: RIMD 0509952, serovar: O157:H7). | The pchA and PpchA is from its organism, the enterohemorrhagic Escherichia coli strain O157:H7 (strain: Sakai, substrain: RIMD 0509952, serovar: O157:H7). | ||
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The PLEE1 is from its organism, the Escherichia coli O157:H7 str. TW14359. | The PLEE1 is from its organism, the Escherichia coli O157:H7 str. TW14359. | ||
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CI repressor protein is known to be derived from E. coli phage (+LVA). | CI repressor protein is known to be derived from E. coli phage (+LVA). | ||
===References=== | ===References=== | ||
Latest revision as of 11:26, 10 August 2023
pchA-PpchA-PLEE1-CI
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 143
Illegal AgeI site found at 609 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 75
Design Notes
Lrp is a leucine-reactive protein expressed by E. coli itself, and exogenous leucine affects the expression of many different operons. When it is necessary to more accurately monitor the content of butyrate in the gut, it is better to ensure that there is enough Lrp protein in the human gut.
Source
The pchA and PpchA is from its organism, the enterohemorrhagic Escherichia coli strain O157:H7 (strain: Sakai, substrain: RIMD 0509952, serovar: O157:H7).
The PLEE1 is from its organism, the Escherichia coli O157:H7 str. TW14359.
CI repressor protein is known to be derived from E. coli phage (+LVA).