Difference between revisions of "Part:BBa K4656009:Design"
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The tetracycline repressor(tetR) is derived from transposon Tn10 (+LVA). | The tetracycline repressor(tetR) is derived from transposon Tn10 (+LVA). | ||
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The lacI repressor and T7 terminator are derived from E. coli (+LVA). | The lacI repressor and T7 terminator are derived from E. coli (+LVA). | ||
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The MazF and MazE are derived from Bacillus subtilis. | The MazF and MazE are derived from Bacillus subtilis. | ||
===References=== | ===References=== |
Latest revision as of 11:24, 10 August 2023
Plac-tetR-Ptet-lacI-MazF
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2237
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
MazF can be characterized in a cell density-dependent manner in E. coli. When the expression of both MazF and MazE genes is turned off (because both are constrained by the same promoter), MazE degrades faster than MazF. If there is nothing to inhibit MazF, then the toxins produced will eventually kill the cells.
Source
The tetracycline repressor(tetR) is derived from transposon Tn10 (+LVA).
The lacI repressor and T7 terminator are derived from E. coli (+LVA).
The MazF and MazE are derived from Bacillus subtilis.