Difference between revisions of "Part:BBa K4656004:Design"

 
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===References===
 
===References===
 +
[1]. Bai, Y. and T.J. Mansell, Production and Sensing of Butyrate in a Probiotic Escherichia coli Strain. Int J Mol Sci, 2020. 21(10).

Revision as of 02:23, 9 August 2023


PLEE1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is better to select the plasmids constructed with different PLEE1 fragments amplified by PCR to select the one with the best expression.


Source

The PLEE1 is from its organism, the Escherichia coli strain O157:H7. Organism: Escherichia coli O157:H7 str. TW14359

References

[1]. Bai, Y. and T.J. Mansell, Production and Sensing of Butyrate in a Probiotic Escherichia coli Strain. Int J Mol Sci, 2020. 21(10).