Difference between revisions of "Part:BBa K4656001:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| − | Since TDC is present in many species, it is best to select the sequence of species with high biological activity in the Escherichia coli system(the one used in our project) during the project to solve the problem of low 5-HT production. | + | Since TDC is present in many species, it is best to select the sequence of species with high biological activity in the Escherichia coli system(the one used in our project) during the project to solve the problem of low 5-HT production. |
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===Source=== | ===Source=== | ||
| − | + | The original sequence of tryptophan decarboxylase(TDC) was derived from the genome of the organism Oryza sativa Japonica Group (Japanese rice). | |
| − | + | Organism: Oryza sativa Japonica Group (Japanese rice) | |
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===References=== | ===References=== | ||
Latest revision as of 09:44, 8 August 2023
TDC
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1248
Illegal BamHI site found at 732
Illegal BamHI site found at 834 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 340
Illegal NgoMIV site found at 775
Illegal NgoMIV site found at 1330
Illegal AgeI site found at 478
Illegal AgeI site found at 1402 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Since TDC is present in many species, it is best to select the sequence of species with high biological activity in the Escherichia coli system(the one used in our project) during the project to solve the problem of low 5-HT production.
Source
The original sequence of tryptophan decarboxylase(TDC) was derived from the genome of the organism Oryza sativa Japonica Group (Japanese rice). Organism: Oryza sativa Japonica Group (Japanese rice)