Difference between revisions of "Part:BBa K4765903"
Siliang Zhan (Talk | contribs) |
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+ | __NOTOC__ | ||
+ | <partinfo>BBa_K4765011 short</partinfo> | ||
+ | We introduced a self-assembly synthetic adhesion system by transfecting this bio-brick into *E. Coli*. The bio-brick is composed of a surface display system(intimin) and the coding sequence of an antigen. The surface display system, which includes a short N-terminal signal peptide to direct its trafficking to the periplasm, a LysM domain for peptidoglycan binding, and a beta-barrel for transmembrane insertion[^1], possess the outer membrane anchoring of the antigen[^2]. The surface-displayed antigen can specifically interact with the nanobody produced by BBk_XXXXXX. In our project, we took full advantage of the Ag-Nb interaction to create a biofilm with a programmable physical structure[^3] | ||
+ | |||
+ | <!-- Add more about the biology of this part here | ||
+ | ===Usage and Biology=== | ||
+ | |||
+ | <!-- --> | ||
+ | <span class='h3bb'>Sequence and Features</span> | ||
+ | <partinfo>BBa_K4765011 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | <!-- Uncomment this to enable Functional Parameter display | ||
+ | ===Functional Parameters=== | ||
+ | <partinfo>BBa_K4765011 parameters</partinfo> | ||
+ | <!-- --> |
Revision as of 09:59, 7 August 2023
MysB codon optimized
We introduced a self-assembly synthetic adhesion system by transfecting this bio-brick into *E. Coli*. The bio-brick is composed of a surface display system(intimin) and the coding sequence of an antigen. The surface display system, which includes a short N-terminal signal peptide to direct its trafficking to the periplasm, a LysM domain for peptidoglycan binding, and a beta-barrel for transmembrane insertion[^1], possess the outer membrane anchoring of the antigen[^2]. The surface-displayed antigen can specifically interact with the nanobody produced by BBk_XXXXXX. In our project, we took full advantage of the Ag-Nb interaction to create a biofilm with a programmable physical structure[^3]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 532