Difference between revisions of "Part:BBa K4119015"

Latest revision as of 04:00, 14 October 2022

PthlA-FNRbindingsite-3

PthlAF3 was modified based on the PthlA constitutive promoter from Clostridium acetobutyricum.A FNR binding site was inserted at the distance of 3 bp from the - 35 region of the PthlA promoter.

Document

Results:

The new promoter thlAF3 has a satisfatory performence. It is inhibited in aerobic condition while strengthed in microaerobic condition.

Fig.2. Expression effect of PthlA and PthlAF3-bs2 at different oxygen concentrations

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 9: / Line 9: @@
 ===Usage and Biology===
-<!-- -->
-<span class='h3bb'>Sequence and Features</span>
-<partinfo>BBa_K4119015 SequenceAndFeatures</partinfo>
@@ Line 18: / Line 15: @@
 <partinfo>BBa_K4119015 parameters</partinfo>
 <!-- -->
+<html lang="en">
+<head>
+    <meta charset="UTF-8">
+    <meta http-equiv="X-UA-Compatible" content="IE=edge">
+    <meta name="viewport" content="width=device-width, initial-scale=1.0">
+    <title>Document</title>
+</head>
+<body>
+    <p style="font-size: 180%; font-weight: bold;">Results:</p>
+    <p>The new promoter thlAF3 has a satisfatory performence. It is inhibited in aerobic condition while strengthed in microaerobic condition.</p>
+    <p><img src="https://static.igem.wiki/teams/4119/wiki/jt-files/h2/img11.png" width="80%" height="80%"></p>
+    <div align="center">
+        <strong>Fig.2. Expression effect of PthlA and PthlAF3-bs2 at different oxygen concentrations</strong>
+    </div>
+</body>
+</html>
+<!-- -->
+<span class='h3bb'>Sequence and Features</span>
+<partinfo>BBa_K4119015 SequenceAndFeatures</partinfo>