Difference between revisions of "Part:BBa K4414001"

 
 
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A nuclear localization signal (NLS) is a short stretch of amino acids that mediates the transport of nuclear proteins into the nucleus. NLS motifs play a key role in this mechanism; 1. typically, deletion of the NLS disrupts nuclear import; and 2. frequently, a non-nuclear protein will be imported into the nucleus if fused to an NLS.
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This nuclear localization sequence（NLS） derived from SV40 directs the protein into the nucleus.
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==Usage and Biology==
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A nuclear localization signal (NLS) is a short stretch of amino acids that mediates the transport of nuclear proteins to the nucleus. The NLS motif plays a key role in this mechanism; (i) normally, deletion of NLS disrupts nuclear import; (ii) if fused to NLS, normally non-nuclear proteins are imported into the nucleus(Cokol et al., 2000) . We obtained this DNA by DNA synthesis.This part can be used in level 1 biological laboratory.
  
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===Usage and Biology===
 
  
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
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===Reference===
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1 .Cokol, M., Nair, R., & Rost, B. (2000). Finding nuclear localization signals. EMBO Reports, 1(5), 411–415. https://doi.org/10.1093/embo-reports/kvd092

Latest revision as of 03:45, 14 October 2022


nuclear localization signal（NLS）

This nuclear localization sequence（NLS） derived from SV40 directs the protein into the nucleus.

Usage and Biology

A nuclear localization signal (NLS) is a short stretch of amino acids that mediates the transport of nuclear proteins to the nucleus. The NLS motif plays a key role in this mechanism; (i) normally, deletion of NLS disrupts nuclear import; (ii) if fused to NLS, normally non-nuclear proteins are imported into the nucleus(Cokol et al., 2000) . We obtained this DNA by DNA synthesis.This part can be used in level 1 biological laboratory.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Reference

1 .Cokol, M., Nair, R., & Rost, B. (2000). Finding nuclear localization signals. EMBO Reports, 1(5), 411–415. https://doi.org/10.1093/embo-reports/kvd092