Difference between revisions of "Part:BBa K4438602"
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+ | __NOTOC__ | ||
+ | <partinfo>BBa_K4438602 short</partinfo> | ||
+ | T6_Target_1 is a single-stranded DNA sequence having 99 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. Its 5’ end has complementary nucleotides for part T6_Trigger_1_midtrigger_phi29 (<partinfo>BBa_K4438601</partinfo>) to bind. The middle region has an ssDNA sense T7 promoter sequence and the 3’ end has an ssDNA sense broccoli light-up aptamer sequence. | ||
+ | |||
+ | |||
+ | ===Usage and Biology=== | ||
+ | This part along with T6_Trigger_1_midtrigger_phi29 (<partinfo>BBa_K4438601</partinfo>) and Aptamer_T6 (<partinfo>BBa_K4438600</partinfo>) can be used for sensing different concentrations of testosterone. | ||
+ | [[File:T--IISER-Tirupati_India--T6_1.png]] | ||
+ | |||
+ | <!-- --> | ||
+ | <span class='h3bb'>Sequence and Features</span> | ||
+ | <partinfo>BBa_K4438602 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | <!-- Uncomment this to enable Functional Parameter display | ||
+ | ===Functional Parameters=== | ||
+ | <partinfo>BBa_K4438602 parameters</partinfo> | ||
+ | <!-- --> | ||
+ | |||
+ | ===References=== | ||
+ | Skouridou, V., Jauset-Rubio, M., Ballester, P., Bashammakh, A. S., El-Shahawi, M. S., Alyoubi, A. O., & O’Sullivan, C. K. (2017). Selection and characterization of DNA aptamers against the steroid testosterone. Microchimica Acta, 184(6), 1631-1639. |
Latest revision as of 03:28, 14 October 2022
T6_Target_1
T6_Target_1 is a single-stranded DNA sequence having 99 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. Its 5’ end has complementary nucleotides for part T6_Trigger_1_midtrigger_phi29 (BBa_K4438601) to bind. The middle region has an ssDNA sense T7 promoter sequence and the 3’ end has an ssDNA sense broccoli light-up aptamer sequence.
Usage and Biology
This part along with T6_Trigger_1_midtrigger_phi29 (BBa_K4438601) and Aptamer_T6 (BBa_K4438600) can be used for sensing different concentrations of testosterone.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Skouridou, V., Jauset-Rubio, M., Ballester, P., Bashammakh, A. S., El-Shahawi, M. S., Alyoubi, A. O., & O’Sullivan, C. K. (2017). Selection and characterization of DNA aptamers against the steroid testosterone. Microchimica Acta, 184(6), 1631-1639.