Difference between revisions of "Part:BBa K4429008:Design"
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | |||
| + | C10 scFv protein sequence obtained from PDB 7CTH [1], reverse translated and codon optimised for E. coli K12 using SnapGene | ||
| + | Removed BioBrick illegal restriction sites using silent mutation in SnapGene | ||
===Source=== | ===Source=== | ||
| − | + | PDB 7CTH [1] | |
- | - | ||
===References=== | ===References=== | ||
| + | [1] https://www.rcsb.org/structure/7CTH | ||
Latest revision as of 03:26, 14 October 2022
scFv C10: EDE1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
C10 scFv protein sequence obtained from PDB 7CTH [1], reverse translated and codon optimised for E. coli K12 using SnapGene
Removed BioBrick illegal restriction sites using silent mutation in SnapGene
Source
PDB 7CTH [1] -