Difference between revisions of "Part:BBa K4429007:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
-
 
  
 +
Ab513 scFv protein sequence obtained from PDB 5AAM [1], reverse translated and codon optimised for E. coli K12 using SnapGene
  
 +
Removed BioBrick illegal restriction sites using silent mutation in SnapGene
  
 
===Source===
 
===Source===
−
 
+
PDB 5AAM [1]
 
-
 
-
  
 
===References===
 
===References===
 +
[1] https://www.rcsb.org/structure/5AAm

Revision as of 03:23, 14 October 2022


scFv513: EDIII


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Ab513 scFv protein sequence obtained from PDB 5AAM [1], reverse translated and codon optimised for E. coli K12 using SnapGene

Removed BioBrick illegal restriction sites using silent mutation in SnapGene

Source

PDB 5AAM [1] -

References

[1] https://www.rcsb.org/structure/5AAm