Difference between revisions of "Part:BBa K4297037"

 
 
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<partinfo>BBa_K4297037 short</partinfo>
 
<partinfo>BBa_K4297037 short</partinfo>
  
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plasmid pYLXP' containing the expressiom cassette of alcohol dehydrogenase ADH4
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The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase ADH4, which can catalyze the reaction of phenylacetaldehyde to 2-PE. The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase PAR8, which can catalyze the reaction of phenylacetaldehyde to 2-PE. The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase PAR2, which can catalyze the reaction of phenylacetaldehyde to 2-PE. Next, we transformed plasmids into Y. lipolytica and perform the whole-cell conversion of phenylacetaldehyde. The whole-cell biocatalytic conversion of 1 g/L phenylacetaldehyde by aforementioned strains gave 2-PE titers of 389.78 mg/L.   
  
  
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        <img src="https://static.igem.wiki/teams/4297/wiki/adh4.jpg" width="50%" style="float:center">
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 03:19, 14 October 2022


pYLXP'-ADH4

The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase ADH4, which can catalyze the reaction of phenylacetaldehyde to 2-PE. The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase PAR8, which can catalyze the reaction of phenylacetaldehyde to 2-PE. The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase PAR2, which can catalyze the reaction of phenylacetaldehyde to 2-PE. Next, we transformed plasmids into Y. lipolytica and perform the whole-cell conversion of phenylacetaldehyde. The whole-cell biocatalytic conversion of 1 g/L phenylacetaldehyde by aforementioned strains gave 2-PE titers of 389.78 mg/L.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1293
    Illegal NotI site found at 1448
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4945
    Illegal BamHI site found at 249
    Illegal XhoI site found at 4316
    Illegal XhoI site found at 4349
    Illegal XhoI site found at 5367
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4806
    Illegal AgeI site found at 950
    Illegal AgeI site found at 4585
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 1284
    Illegal BsaI site found at 4588
    Illegal BsaI.rc site found at 255
    Illegal BsaI.rc site found at 4582
    Illegal BsaI.rc site found at 5651