Difference between revisions of "Part:BBa K4166010"

Latest revision as of 03:09, 14 October 2022

RFP under Lac promoter control with a double inverter (Tet, cI)

To control leaky expression, we inserted a double inverter into the lac promoter. This should change the dynamics of expression enough to limit the problem, while still allowing for induction with IPTG.

Alma 2022 - Leaky vs. Induced Expression

We sought to test our double inverter system (see the model on our Wiki). So we streaked this culture and BBa_J04450 side by side on some agar plates, either with 0 or 100uM IPTG (to induce expression).

Consistent with our modelling, we found no leaky expression, but no significant induction as well.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 2608
Illegal AgeI site found at 2720
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 1302

@@ Line 4: / Line 4: @@
 To control leaky expression, we inserted a double inverter into the lac promoter. This should change the dynamics of expression enough to limit the problem, while still allowing for induction with IPTG.
+=== Alma 2022 - Leaky vs. Induced Expression ===
+We sought to test our double inverter system (see the model on our Wiki). So we streaked this culture and BBa_J04450 side by side on some agar plates, either with 0 or 100uM IPTG (to induce expression).
+[[File:T--Alma--dblinvplate.png|650px|center]]
+Consistent with our modelling, we found no leaky expression, but no significant induction as well.
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