Difference between revisions of "Part:BBa K4438604"

 
 
(4 intermediate revisions by 2 users not shown)
Line 1: Line 1:
 +
__NOTOC__
 +
<partinfo>BBa_K4438604 short</partinfo>
  
 +
T6_Target_2 is single-stranded DNA sequence having 96 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. Its 5’ end has complementary nucleotides for part T6_Trigger_2_phi29 (BBa_K4438601) to bind. The middle region has the ssDNA sense T7 promoter sequence and the 3’ end has the ssDNA sense broccoli light-up aptamer sequence.
 +
 +
 +
 +
===Usage and Biology===
 +
This part along with T6_Trigger_2_phi29 (BBa_K4438601) and Aptamer_T6 (BBa_K4438600) can used for sensing different concentrations of testosterone.
 +
[[File:T--IISER-Tirupati_India--T6_2.png]]
 +
 +
<!-- -->
 +
<span class='h3bb'>Sequence and Features</span>
 +
<partinfo>BBa_K4438100 SequenceAndFeatures</partinfo>
 +
 +
 +
<!-- Uncomment this to enable Functional Parameter display
 +
===Functional Parameters===
 +
<partinfo>BBa_K4438604 parameters</partinfo>
 +
<!-- -->
 +
 +
===References===
 +
Skouridou, V., Jauset-Rubio, M., Ballester, P., Bashammakh, A. S., El-Shahawi, M. S., Alyoubi, A. O., & O’Sullivan, C. K. (2017). Selection and characterization of DNA aptamers against the steroid testosterone. Microchimica Acta, 184(6), 1631-1639.

Latest revision as of 03:03, 14 October 2022

T6_Target_2

T6_Target_2 is single-stranded DNA sequence having 96 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. Its 5’ end has complementary nucleotides for part T6_Trigger_2_phi29 (BBa_K4438601) to bind. The middle region has the ssDNA sense T7 promoter sequence and the 3’ end has the ssDNA sense broccoli light-up aptamer sequence.


Usage and Biology

This part along with T6_Trigger_2_phi29 (BBa_K4438601) and Aptamer_T6 (BBa_K4438600) can used for sensing different concentrations of testosterone. T--IISER-Tirupati India--T6 2.png

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 51
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 51
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 51
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 51
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Skouridou, V., Jauset-Rubio, M., Ballester, P., Bashammakh, A. S., El-Shahawi, M. S., Alyoubi, A. O., & O’Sullivan, C. K. (2017). Selection and characterization of DNA aptamers against the steroid testosterone. Microchimica Acta, 184(6), 1631-1639.